Advanced Time-Correlated Single Photon Counting Applications

✍ Scribed by Wolfgang Becker (eds.)

• Publisher: Springer International Publishing
• Year: 2015
• Tongue: English
• Leaves: 639
• Series: Springer Series in Chemical Physics 111
• Edition: 1
• Category: Library

✦ Synopsis

This book is an attempt to bridge the gap between the instrumental principles of multi-dimensional time-correlated single photon counting (TCSPC) and typical applications of the technique. Written by an originator of the technique and by sucessful users, it covers the basic principles of the technique, its interaction with optical imaging methods and its application to a wide range of experimental tasks in life sciences and clinical research.

The book is recommended for all users of time-resolved detection techniques in biology, bio-chemistry, spectroscopy of live systems, live cell microscopy, clinical imaging, spectroscopy of single molecules, and other applications that require the detection of low-level light signals at single-photon sensitivity and picosecond time resolution.

✦ Table of Contents

Front Matter....Pages i-xxiii
Introduction to Multi-dimensional TCSPC....Pages 1-63
TCSPC FLIM with Different Optical Scanning Techniques....Pages 65-117
Fluorescence Lifetime Imaging (FLIM): Basic Concepts and Recent Applications....Pages 119-188
Determination of Intracellular Chloride Concentrations by Fluorescence Lifetime Imaging....Pages 189-211
Calcium Imaging Using Transient Fluorescence-Lifetime Imaging by Line-Scanning TCSPC....Pages 213-224
Imaging Cell and Tissue O 2 by TCSPC-PLIM....Pages 225-247
FRET Microscopy: Basics, Issues and Advantages of FLIM-FRET Imaging....Pages 249-276
Monitoring HIV-1 Protein Oligomerization by FLIM FRET Microscopy....Pages 277-307
Unraveling the Rotary Motors in F o F 1 -ATP Synthase by Time-Resolved Single-Molecule FRET....Pages 309-338
Partitioning and Diffusion of Fluorescently Labelled FTY720 in Resting Epithelial Cells....Pages 339-355
Probing Microsecond Reactions with Microfluidic Mixers and TCSPC....Pages 357-384
An Introduction to Interpreting Time Resolved Fluorescence Anisotropy Curves....Pages 385-406
Time-Resolved Spectroscopy of NAD(P)H in Live Cardiac Myocytes....Pages 407-434
Fluorescence Lifetime Measurements of NAD(P)H in Live Cells and Tissue....Pages 435-456
Fluorescence Lifetime Imaging of the Skin....Pages 457-508
Fluorescence Lifetime Imaging in Ophthalmology....Pages 509-540
Dynamic Mapping of the Human Brain by Time-Resolved NIRS Techniques....Pages 541-559
Time-Domain Diffuse Optical Imaging of Tissue by Non-contact Scanning....Pages 561-585
Breast Monitoring by Time-Resolved Diffuse Optical Imaging....Pages 587-611
Back Matter....Pages 613-624

✦ Subjects

Physical Chemistry; Optics and Electrodynamics; Atomic/Molecular Structure and Spectra; Measurement Science and Instrumentation; Signal, Image and Speech Processing