Advanced Techniques in Soil Microbiology

✍ Scribed by R. Jeewon, K. D. Hyde (auth.), Prof. Dr. Ajit Varma, Prof. Dr. Ralf Oelmüller (eds.)

• Publisher: Springer-Verlag Berlin Heidelberg
• Year: 2007
• Tongue: English
• Leaves: 438
• Series: Soil Biology 11
• Edition: 1
• Category: Library

✦ Synopsis

"Advanced Techniques in Soil Microbiology" presents a wide range of biotechnological methods for application in soil microbiology analysis. These include all essential methods involving molecular biology, immunology, microbiology, and structural biology, such as transcriptome analysis, RNAi technology, molecular matchmaking, RAPD, T-RFLP and FT/MS.

The techniques and procedures have been selected with the aim of offering practical guides for immediate use in the laboratory. The systems investigated range from individual molecules and cells to entire eukaryotic organisms, with a focus on bacteria, fungi, mycorrhiza, and higher plants. This volume of state-of-the-art, practice oriented methods will be of great use both to the first-timer and to the experienced scientist.

✦ Table of Contents

Front Matter....Pages I-XXVIII
Detection and Diversity of Fungi from Environmental Samples: Traditional Versus Molecular Approaches....Pages 1-15
Functional Genomic Approaches for Mycorrhizal Research....Pages 17-33
Automated Fluoroscence Sequencing and Troubleshooting....Pages 35-51
mRNA Quantitation Using Real Time PCR....Pages 53-72
Laboratory Practice for the Production of Polyclonal and Monoclonal Antibodies....Pages 73-91
Modern Techniques for Analyzing Immunological Responses....Pages 93-109
Transcriptome Analysis....Pages 111-131
RNAi Technology: a Tool for Functional Validation of Novel Genes....Pages 133-144
Molecular Matchmaking: Techniques for Biomolecular Interactions....Pages 145-153
Environmental Proteomics: Extraction and Identification of Protein in Soil....Pages 155-166
DGGE and RISA Protocols for Microbial Community Analysis in Soil....Pages 167-180
Soil Microbial Community Structure and Function Assessed by FAME, PLFA and DGGE — Advantages and Limitations....Pages 181-200
Measurement of Microbial Biomass and Activity in Soil....Pages 201-211
Immuno-Technology for the Localization of Acid Phosphatase Using Native Gel Bands in Piriformospora indica and Other Soil Microorganisms....Pages 213-235
Use of Short Oligonucleotide Primers in Random Amplified Polymorphic DNA Techniques for Species Identification....Pages 237-246
Co-Cultivation with Sebacinales....Pages 247-270
Quantitative Histochemistry: a Forgotten Tool with New Applications....Pages 271-279
Ion Cyclotron Resonance Fourier Transform Mass Spectrometry for Non-Targeted Metabolomics of Molecular Interactions in the Rhizosphere....Pages 281-293
Application of Terminal-Restriction Fragment Length Polymorphism for MolecularAnalysis of Soil Bacterial Communities....Pages 295-305
Molecular Symbiotic Analysis Between Arabiopsis thaliana and Piriformospora indica ....Pages 307-318
Biophysical Phenomics Reveals Functional Building Blocks of Plants Systems Biology: a Case Study for the Evaluation of the Impact of Mycorrhization with Piriformospora indica ....Pages 319-341
Analysis of the Plant Protective Potential of the Root Endophytic Fungus Piriformospora indica in Cereals....Pages 343-354
Members of Sebacinales Confer Resistance Against Heavy Metal Stress in Plants....Pages 355-361
Screening of Plant Growth-Promoting Rhizobacteria....Pages 363-375
Research Methods in Arbuscular Mycorrhizal Fungi....Pages 377-396
Field Trials of Bioinoculants....Pages 397-413
Back Matter....Pages 415-427

✦ Subjects

Microbiology; Plant Sciences; Agriculture; Biotechnology; Soil Science & Conservation