Advanced Techniques in Biological Electron Microscopy III

✍ Scribed by J. Frank, M. Radermacher (auth.), James K. Koehler Ph. D. (eds.)

• Publisher: Springer-Verlag Berlin Heidelberg
• Year: 1986
• Tongue: English
• Leaves: 288
• Edition: 1
• Category: Library

✦ Synopsis

This volume is a continuation of two prior books on advanced electron microscope techniques. The purpose of this series has been to provide in­ depth analyses of methods which are considered to be at the leading edge of electron microscopic research procedures with applications in the biological sciences. The mission of the present volume remains that of a source book for the research practitioner or advanced student, especially one already well versed in basic electron optical methods. It is not meant to provide in­ troductory material, nor can this modest volume hope to cover the entire spectrum of advanced technology now available in electron microscopy. In the past decade, computers have found their way into many research laboratories thanks to the enormous increase in computing power and stor­ age available at a modest cost. The ultrastructural area has also benefited from this expansion in a number of ways which will be illustrated in this volume. Half of the contributions discuss technologies that either directly or indirectly make extensive use of computer methods.

✦ Table of Contents

Front Matter....Pages I-XIII
Three-Dimensional Reconstruction of Nonperiodic Macromolecular Assemblies from Electron Micrographs....Pages 1-72
High Resolution Biological X-Ray Microanalysis of Diffusable Ions....Pages 73-100
Metal Deposition by High-Energy Sputtering for High Magnification Electron Microscopy....Pages 101-166
Computer Programs for Biological Stereology....Pages 167-200
A Guide to Fracture Label: Cytochemical Labeling of Freeze-Fractured Cells....Pages 201-227
The Preparation of Colloidal Gold Probes and Their Use as Marker in Electron Microscopy....Pages 229-271
Back Matter....Pages 273-278

✦ Subjects

Cell Biology;Biochemistry, general;Computer Appl. in Life Sciences;Atomic, Molecular, Optical and Plasma Physics