Advanced Methods in Protein Sequence Determination

✍ Scribed by Professor Saul B. Needleman (auth.)

• Publisher: Springer-Verlag Berlin Heidelberg
• Year: 1977
• Tongue: English
• Leaves: 200
• Series: Molecular Biology, Biochemistry and Biophysics / Molekularbiologie, Biochemie und Biophysik 25
• Edition: 1
• Category: Library

✦ Synopsis

Confusion now hath made his masterpiece Macbeth II iii 72 Whence and what are those execrable shape? Paradise Lost Ib 1 681 Confusion worse confounded Paradise Lost Ib 1 995 When the manuscript for the first part of this book was proposed, it was anticipated that the discussion of the entire field of protein se­ quencing could be covered in a single volume - from purification and characterization of the protein through fragmentation by chemical or enzymic means and, finally, to reassembly of the identified individual peptides into the reconstructed total sequence. It soon became evident that this would not be possible. While the intent was to restrict the expose of procedures only to that information concerned with "hands on" wet chemistry, it became apparent that a thorough presentation would require, in addition, a discussion of certain instrumental and more theoretical approaches not included in the first volume. Furthermore, the entire understanding of the field of protein sequencing has advanced appreciably since the inception of this book. The purpose of the first volume was to provide practical information in sufficient detail to permit the researcher to undertake the actual sequencing procedures in his own laboratory.

✦ Table of Contents

Front Matter....Pages I-XII
Step-wise Degradation of Peptides Attached to Solid Supports....Pages 1-20
Coupling Methods and Strategies in Solid-Phase Sequencing....Pages 21-37
Sequencing Peptides and Proteins Lacking Free α-Amino Groups....Pages 38-54
Use of Antibody in the Study of Protein Structure....Pages 55-67
Polarization of Light and Protein Structure....Pages 68-74
The X-ray Crystallography Technique in Protein Sequencing....Pages 75-122
Amino Acid Sequence Determination by Mass Spectrometry....Pages 123-148
Peptide Sequence Analysis by Nuclear Magnetic Resonance Spectroscopy....Pages 149-171
Back Matter....Pages 172-191

✦ Subjects

Life Sciences, general; Biomedicine general