Advanced Fluorescence Reporters in Chemistry and Biology III: Applications in Sensing and Imaging

✍ Scribed by Alexander P. Demchenko, Semen O. Yesylevskyy (auth.), Alexander P. Demchenko (eds.)

• Publisher: Springer-Verlag Berlin Heidelberg
• Year: 2011
• Tongue: English
• Leaves: 364
• Series: Springer Series on Fluorescence 113
• Edition: 1
• Category: Library

✦ Synopsis

The key element of any fluorescence sensing or imaging technology is the fluorescence reporter, which transforms the information on molecular interactions and dynamics into measurable signals of fluorescence emission. This book, written by a team of frontline researchers, demonstrates the broad field of applications of fluorescence reporters, starting from nanoscopic properties of materials, such as self-assembled thin films, polymers and ionic liquids, through biological macromolecules and further to living cell, tissue and body imaging. Basic information on obtaining and interpreting experimental data is presented and recent progress in these practically important areas is highlighted. The book is addressed to a broad interdisciplinary audience.

✦ Table of Contents

Front Matter....Pages i-x
Front Matter....Pages 1-1
Interfacial Behavior of Fluorescent Dyes....Pages 3-62
Front Matter....Pages 63-63
Fluorescence Probing of the Physicochemical Characteristics of the Room Temperature Ionic Liquids....Pages 65-89
Fluorescence Spectroscopy in Polymer Science....Pages 91-117
Fluorescence Probing in Structurally Anisotropic Materials....Pages 119-158
Front Matter....Pages 159-159
Optimized Dyes for Protein and Nucleic Acid Detection....Pages 161-199
Functional Nucleic Acids for Fluorescence-Based Biosensing Applications....Pages 201-221
Front Matter....Pages 223-223
Covalent Labeling of Biomolecules in Living Cells....Pages 225-261
Tetracysteine and Bipartite Tags for Biarsenical Organic Fluorophores....Pages 263-295
Labeling of Oligohistidine-Tagged Proteins....Pages 297-310
Front Matter....Pages 311-311
In Vivo Imaging of Vascular Targets Using Near-Infrared Fluorescent Probes....Pages 313-328
Whole-Body Imaging of Hematopoietic and Cancer Cells Using Near-Infrared Probes....Pages 329-346
Back Matter....Pages 347-352

✦ Subjects

Analytical Chemistry;Molecular Medicine;Medical Biochemistry;Biophysics and Biological Physics;Optical and Electronic Materials;Biological Techniques