Advanced Flow Cytometry: Applications in Biological Research

✍ Scribed by L. Scott Cram (auth.), R. C. Sobti, Awtar Krishan (eds.)

• Publisher: Springer Netherlands
• Year: 2003
• Tongue: English
• Leaves: 113
• Edition: 1
• Category: Library

✦ Synopsis

Flow cytometry has rapidly evolved into a technique for rapid analysis of DNA content, cellular marker expression and electronic sorting of cells of interest for further investigations. Flow cytometers are being extensively used for monitoring of cellular DNA content, phenotype expression, drug transport, calcium flux, proliferation and apoptosis. Phenotypic analysis of marker expression in leukemic cells has become an important tool for diagnostic and therapeutic monitoring of patients. Recent studies have explored the use of flow cytometry for monitoring hormone receptor expression in human solid tumors and for studies in human genomics. Contributions in the current volume are based on presentations made at the First Indo-US workshop on Flow Cytometry in which experts from USA, UK and India discussed applications of flow cytometry in biological and medical research. This book will be of interest to post graduates and researchers in the fields of pathology, cytology, cell biology and molecular biology.

✦ Table of Contents

Front Matter....Pages i-iv
Flow cytometry, an overview....Pages 1-9
High resolution flow cytometric analysis of electronic nuclear volume and DNA content in normal and abnormal human tissue....Pages 11-18
Analysis of heterogeneous red cell populations by flow cytometry....Pages 19-25
Chromosome sorting and genomics....Pages 27-35
Analysis of intracellular cytokines using flowcytometry....Pages 37-40
Cell array coupled with laser scanning cytometry allows easy analysis of changes in cyclin expression during the cell cycle....Pages 41-47
Flow cytometry and its application in small animal oncology....Pages 49-54
Flow cytometric monitoring of drug resistance in human tumor cells....Pages 55-60
Androgen receptor expression in archival human breast tumors....Pages 61-64
Membrane oxidative damage and apoptosis in cervical carcinoma cells of patients after radiation therapy....Pages 65-68
Nitric oxide mediated modulation of free radical generation response in the rat polymorphonuclear leukocytes: A flowcytometric study....Pages 69-76
Flowcytometric detection of PNH defect and response to therapy in aplastic anemia patients....Pages 77-78
Host-pathogen interactions: leukocyte phagocytosis and associated sequelae....Pages 79-90
Flow cytometric techniques for the detection of microorganisms....Pages 91-97
A flow cytometric approach to assess phytoplankton respiration....Pages 99-106
Cellular and serological markers of disease activity in Indian patients with HIV/AIDS....Pages 107-114

✦ Subjects

Oncology; Pathology; Life Sciences, general; Human Genetics; Medical Microbiology