Adsorption/desorption of human serum albumin at the surface of poly(lactic acid) nanoparticles prepared by a solvent evaporation process
✍ Scribed by Verrecchia, T. ;Huve, P. ;Bazile, D. ;Veillard, M. ;Spenlehauer, G. ;Couvreur, P.
- Publisher
- John Wiley and Sons
- Year
- 1993
- Tongue
- English
- Weight
- 996 KB
- Volume
- 27
- Category
- Article
- ISSN
- 0021-9304
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✦ Synopsis
Biocompatible and biodegradable nanoparticles of poly-(lactic acid) (100% L-lactic units = PLA) were prepared by an emulsion, microfluidization, and solvent evaporation method using human serum albumin (HSA) as a surface agent. A radiolabeling technique was employed to quantify the serum albumin bound to the nanoparticles and to measure its desorption kinetics in various media at 22°C and 37°C (phosphate buffer pH 7.4, serum albumin 40 g/L in phosphate buffer pH 7.4 and fetal calf serum). The amount of serum albumin bound to the nanoparticles was found to be a linear function of 1/D (where D is the nanoparticle mean diameter) and was related to the total developed area of the nanoparticles. The adsorption/ desorption behavior of serum albumin at the surface of the nanoparticles suggested a multilayer adsorption model. Moreover, a part of the serum albumin molecules was irreversibly bound regardless of the incubation conditions. Consequently, the classical Langmuirian theories of equilibria could not be applied.