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Adsorption of Nerve Growth Factor onto Surfaces Implications for the Assay in Tissue Culture

✍ Scribed by Frederick L. Pearce; Derek V. BANTHORPE; Janet M. Cook; Charles A. VERNON

Book ID
115113486
Publisher
John Wiley and Sons
Year
1973
Tongue
English
Weight
752 KB
Volume
32
Category
Article
ISSN
1432-1327

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## Abstract When single‐cell suspensions prepared from embroyonic day 8 (E8) chick sensory ganglia are incubated with nerve growth factor (NGF), anti‐NGF antiserum, and complement, an NGF‐dependent cytotoxic kill of 20 (Β±3)% of the ganglia cells is observed. This percentage is increased by a factor