✦ LIBER ✦
Adsorption of human IgG on Cu2+-immobilized cellulose affinity membrane: Preliminary study
✍ Scribed by Hari, P. R. ;Paul, Willi ;Sharma, Chandra P.
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 173 KB
- Volume
- 50
- Category
- Article
- ISSN
- 0021-9304
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✦ Synopsis
Immobilized metal ion affinity chromatography (IMAC) is widely used. Transition metal ions have a high affinity to some peptide sequences. We have studied the selective adsorption of human IgG from a mixture of albumin, ␥-globulin, fibrinogen, and IgG onto Cu 2+ ionimmobilized cellulose membrane. Although Cu 2+ ligand is selective to IgG, in general ␥-globulins also are adsorbed. The simplicity and lower cost of Cu 2+ ion-immobilized cellulose membranes may be useful for removing IgG from blood.