Adsorption of commercially prepared trypsin using a membrane support material

✍ Scribed by A. Carter; J. A. Howell

• Book ID: 104648617
• Publisher: Springer-Verlag
• Year: 1987
• Tongue: English
• Weight: 132 KB
• Volume: 1
• Category: Article
• ISSN: 0951-208X
• DOI: 10.1007/bf00159332

✦ Synopsis

The ability of a recently developed affinity membrane to adsorb commercially prepared trypsin was investigated.

Several buffered solutions of trypsin which varied in their initial concentrations from 62.5 mg/Q to 1,000 mg/Q, were passed through a stacked bed of seven membranes;

dry wt 350 mgs.

The adsorbed protein was eluted using acetic acid; 2.2 mgs to 5.3 mgs of trypsin was desorbed.

The adsorption capacity tended to a maximum of 16 mg Ig dry wt when the initial feed concentration of trypsin was 1 g/Q.

There was no loss in enzyme activity after desorption; 11,500 K-J + 500 IU.