Adsorption of Catalase on Hydroxyapatite

✍ Scribed by Allal Barroug; Etienne Lernoux; Jacques Lemaitre; Paul G. Rouxhet

• Publisher: Elsevier Science
• Year: 1998
• Tongue: English
• Weight: 98 KB
• Volume: 208
• Category: Article
• ISSN: 0021-9797
• DOI: 10.1006/jcis.1998.5759

✦ Synopsis

The adsorption of catalase on calcium hydroxyapatite is reported in this study. In all the solutions investigated, catalase adsorption takes place under conditions where the adsorbent and the adorbate are both negatively charged and is accompanied by the release of phosphate ions. This suggests that electrostatic attraction does not play a leading role in the adsorption process. However, electrostatic repulsion does play a role as evidenced by the influence of the solution composition on the maximum amount adsorbed. In fact, the amount adsorbed increases when the surface charge tends to be less negative (decrease of pH and phosphate concentration, presence of calcium) or when the electrical charges are screened as a result of the ionic strength increase. This was confirmed by the electrophoretic mobility which becomes more negative after protein adsorption. The rate of catalase adsorption is extremely low; the time required to reach a stationary concentration is 60 and 125 h in potassium nitrate and phosphate solution, respectively. The reversibility of catalase was examined with respect to changes in the bulk solution concentration. No significant desorption was obtained after several days of observation. The adsorption, desorption, and kinetic aspects have been discussed in relation to changes in the the protein structure upon interaction and to the microstructural characteristics of the mineral.