Adsorption of Bovine Serum Albumin onto Polystyrene Latex Particles Bearing Saccharidic Moieties

✍ Scribed by Javier Revilla; Abdelhamid Elaı̈ssari; Pascal Carriere; Christian Pichot

• Publisher: Elsevier Science
• Year: 1996
• Tongue: English
• Weight: 187 KB
• Volume: 180
• Category: Article
• ISSN: 0021-9797
• DOI: 10.1006/jcis.1996.0319

✦ Synopsis

texes bearing aldehyde (7), carboxylic (6-8), sulfhydryl The adsorption of bovine serum albumin (BSA) onto polysty- (9), or amino groups (10) to provide covalent immobilizarene latexes bearing various amounts of sugar moieties has been tion of the proteins have been prepared. However, it was investigated as a function of pH and ionic strength and the results reported that nonspecific adsorption could affect such fixawere compared to those for bare polystyrene latexes having negation and methods have been extensively developed with a tive surface charges. The functionalized latexes were produced by view to controlling the hydrophilic-lipophilic balance at the seeded copolymerization of (0.3 mm) liposaccharidic monomer water-polymer interface. Emulsifier-free emulsion copolyonto polystyrene particles obtained by soap-free emulsion polymerization of styrene in the presence of a polar monomer merization of styrene using potassium persulfate as initiator. At has appeared as a versatile method.

first, the electrophoretic mobility behavior of the various latexes was examined as a function of pH: a significant decrease was Depending upon experimental conditions, the hydrophilic observed in the case of saccharide-containing latex particles commonomer can be predominantly incorporated at the particle pared to the bare particles. The adsorption of BSA onto these surface either as small spots or as a hydrophilic shell forming latexes exhibited a reduced amount of adsorbed BSA for those a hairy layer. This was thoroughly in evidence in the cases of latex particles bearing saccharide groups. This adsorbed amount acrylamide (3), acrylic acid (5), hydroxyethyl methacrylate depends on the yield of saccharidic monomer incorporated onto (5), etc. In addition, one outstanding property of such lathe surfaces of the latex particles.