## Abstract Highly charged water‐soluble conjugates of amyloglucosidase with a copolymer of ethylene‐maleic acid or styrene‐maleic acid were prepared and adsorbed on DEAE‐cellulose and other cationic resins. Negatively charged enzyme conjugates were also obtained by means of succinylation. The poly
Adsorption of amyloglucosidase on inorganic carriers
✍ Scribed by B. Solomon; Y. Levin
- Publisher
- John Wiley and Sons
- Year
- 1975
- Tongue
- English
- Weight
- 434 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0006-3592
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✦ Synopsis
Abstract
A simple method for immobilizing amyloglucosidase by adsorption on inorganic carriers is described. Amyloglucosidase was adsorbed on acid‐activated molecular sieve and on alumina. The immobilized enzyme preparations exhibited 50–100% of the initial activity and possessed high temperature stability. A prolonged working life span was achieved, which could possibly satisfy requirements for industrial application.
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Gel permeation chromatography (GPC) calibration curves log hydrodynamic volume versus elution volumes were obtained from a series of single and mixed eluents for polystyrene on inorganic carriers. The observed calibration shifts were interpreted as a result of adsorption and partition effects on elu