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Adsorption Mechanism of Polypeptides and Their Location at Hydrophobic Interfaces

✍ Scribed by Tobias Pirzer; Thorsten Hugel

Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
398 KB
Volume
10
Category
Article
ISSN
1439-4235

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✦ Synopsis

Abstract

The adhesion of polypeptides and proteins at interfaces is important for a wide variety of systems—from the adhesion onto vessels during their production process over functional coatings to protein–membrane interactions in single cells. Herein, we apply an atomic‐force‐microscopy‐based single‐molecule method and poly‐D‐tyrosine to determine the adhesion strength and location of polypeptides at interfaces. Surprisingly, the support (solid, liquid or gas) hardly influences the adhesion in an aqueous environment, while the addition of ethanol to the solvent cuts the adhesion in half. These findings allow us to propose that the adsorbed polypeptide spans over both the depletion layer and the hydrophobic hydration layer to facilitate a compensation mechanism between dispersive and hydration forces.

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