Adsorption and Structural Change of β-Lactoglobulin at the Diacylglycerol−Water Interface
✍ Scribed by Sakuno, Mirian M.; Matsumoto, Shinya; Kawai, Shigeru; Taihei, Koseki; Matsumura, Yasuki
- Book ID
- 126070296
- Publisher
- American Chemical Society
- Year
- 2008
- Tongue
- English
- Weight
- 166 KB
- Volume
- 24
- Category
- Article
- ISSN
- 0743-7463
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Dynamics of adsorption of 14 C radiolabeled -lactoglobulin and ␣-lactalbumin at the air-water interface was investigated through the measurement of surface pressure () and surface concentration (⌫) via a radiotracer technique. Adsorption was diffusion controlled at short times, the rates of increas
Detailed insight can be obtained from proteins at and near the air-water interface using external reflection IR and circular dichroism techniques. Besides information on local protein concentrations and surface layer thickness, it is shown that beta-lactoglobulin displays a limited unfolding at the