Adsorption and enzyme (β-galactosidase and α-chymotrypsin): Immobilization properties of gel fiber prepared by the gel formation of cellulose acetate and titanium iso-propoxide
✍ Scribed by Youichi Kurokawa; Keiichiro Suzuki; Yuko Tamai
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 118 KB
- Volume
- 59
- Category
- Article
- ISSN
- 0006-3592
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✦ Synopsis
We prepared a new composite gel fiber by the gel formation of cellulose acetate and titanium isopropoxide. The fiber is harder than alginate gel; it is also stable in common solvents, phosphate solution, and electrolyte solutions over a wide range of pH from 3 to 10. The fiber shows amphoretic adsorption properties depending on pH, namely, it acts anionic with decreasing pH and cationic with increasing pH. However, the fiber had no adsorption property for a pyrogen endotoxin. The -galactosidase and ␣-chymotrypsin not retained in alginate gel were immobilized on the fibers by this method. The pH, temperature, and repeated run stabilities of the immobilized enzyme were compared to those of the native one.