ADP-ribosylation and NAD+ Utilizing Enzymes: Methods and Protocols

✍ Scribed by Paul Chang

Publisher: Springer New York;Humana Press
Year: 2018
Tongue: English
Leaves: 410
Series: Methods in Molecular Biology 1813
Edition: 1st ed.
Category: Library

No coin nor oath required. For personal study only.

✦ Synopsis

This volume focuses on mono-ADP-ribosylation and enzymes that use NAD+ including Sirtuins, PARPs, and bacterial and eukaryotic ADP-ribosyltransferases. The chapters in this book are organized into eight parts, and offer detailed descriptions of key protocols used to study topics such as in vitro techniques for ADP-ribosylation substrate identification; biochemical and biophysical assays of PAR-WWE domain interactions; monitoring expression and enzyme activity of ecto-ARTCs; HPLC-based enzymes assays for Sirtuins; and identifying target RNAs of PARPs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, ADP-ribosylation and NAD+ Utilizing Enzymes: Methods and Protocols is a valuable resource for anyone interested in this developing and expanding field.

✦ Table of Contents

Front Matter ....Pages i-xiv
Front Matter ....Pages 1-1
Vitamin B3 in Health and Disease: Toward the Second Century of Discovery (Myron K. Jacobson, Elaine L. Jacobson)....Pages 3-8
Front Matter ....Pages 9-9
Monitoring Poly(ADP-Ribosyl)ation in Response to DNA Damage in Live Cells Using Fluorescently Tagged Macrodomains (Rebecca Smith, Gyula Timinszky)....Pages 11-24
In Vitro Techniques for ADP-Ribosylated Substrate Identification (Giovanna Grimaldi, Giuliana Catara, Carmen Valente, Daniela Corda)....Pages 25-40
Assessment of Intracellular Auto-Modification Levels of ARTD10 Using Mono-ADP-Ribose-Specific Macrodomains 2 and 3 of Murine Artd8 (Mareike Bütepage, Sarah Krieg, Laura Eckei, Jinyu Li, Giulia Rossetti, Patricia Verheugd et al.)....Pages 41-63
Biochemical and Biophysical Assays of PAR-WWE Domain Interactions and Production of iso-ADPr for PAR-Binding Analysis (Zhizhi Wang, Wenqing Xu)....Pages 65-73
Front Matter ....Pages 75-75
Assays for NAD+-Dependent Reactions and NAD+ Metabolites (Michael B. Schultz, Yuancheng Lu, Nady Braidy, David A. Sinclair)....Pages 77-90
Generating Protein-Linked and Protein-Free Mono-, Oligo-, and Poly(ADP-Ribose) In Vitro (Ken Y. Lin, Dan Huang, W. Lee Kraus)....Pages 91-108
Methods to Study TCDD-Inducible Poly-ADP-Ribose Polymerase (TIPARP) Mono-ADP-Ribosyltransferase Activity (David Hutin, Giulia Grimaldi, Jason Matthews)....Pages 109-124
Dictyostelium as a Model to Assess Site-Specific ADP-Ribosylation Events (Anna-Lena Kolb, Duen-Wei Hsu, Ana B. A. Wallis, Seiji Ura, Alina Rakhimova, Catherine J. Pears et al.)....Pages 125-148
Mono-ADP-Ribosylation Catalyzed by Arginine-Specific ADP-Ribosyltransferases (Linda A. Stevens, Joel Moss)....Pages 149-165
Monitoring Expression and Enzyme Activity of Ecto-ARTCs (Stephan Menzel, Sahil Adriouch, Peter Bannas, Friedrich Haag, Friedrich Koch-Nolte)....Pages 167-186
ADP-Ribosyl-Acceptor Hydrolase Activities Catalyzed by the ARH Family of Proteins (Masato Mashimo, Joel Moss)....Pages 187-204
Mono-ADP-Ribosylhydrolase Assays (Jeannette Abplanalp, Ann-Katrin Hopp, Michael O. Hottiger)....Pages 205-213
Hydrolysis of ADP-Ribosylation by Macrodomains (Melanija Posavec Marjanovic´, Gytis Jankevicius, Ivan Ahel)....Pages 215-223
HPLC-Based Enzyme Assays for Sirtuins (Jun Young Hong, Xiaoyu Zhang, Hening Lin)....Pages 225-234
Front Matter ....Pages 235-235
Small-Molecule Screening Assay for Mono-ADP-Ribosyltransferases (Teemu Haikarainen, Sudarshan Murthy, Mirko M. Maksimainen, Lari Lehtiö)....Pages 237-244
A Simple, Sensitive, and Generalizable Plate Assay for Screening PARP Inhibitors (Ilsa T. Kirby, Rory K. Morgan, Michael S. Cohen)....Pages 245-252
Front Matter ....Pages 253-253
Nonlocalized Searching of HCD Data for Fast and Sensitive Identification of ADP-Ribosylated Peptides (Thomas Colby, Juan José Bonfiglio, Ivan Matic)....Pages 255-269
Quantitative Determination of MAR Hydrolase Residue Specificity In Vitro by Tandem Mass Spectrometry (Robert Lyle McPherson, Shao-En Ong, Anthony K. L. Leung)....Pages 271-283
Front Matter ....Pages 285-285
Detection of ADP-Ribosylating Bacterial Toxins (Chen Chen, Joseph T. Barbieri)....Pages 287-295
Preparation of Recombinant Alphaviruses for Functional Studies of ADP-Ribosylation (Rachy Abraham, Robert Lyle McPherson, Easwaran Sreekumar, Anthony K. L. Leung, Diane E. Griffin)....Pages 297-316
Monitoring the Sensitivity of T Cell Populations Towards NAD+ Released During Cell Preparation (Björn Rissiek, Marco Lukowiak, Friedrich Haag, Tim Magnus, Friedrich Koch-Nolte)....Pages 317-326
Identifying Target RNAs of PARPs (Florian J. Bock, Paul Chang)....Pages 327-341
Front Matter ....Pages 343-343
ADPr-Peptide Synthesis (Hans A. V. Kistemaker, Jim Voorneveld, Dmitri V. Filippov)....Pages 345-369
Identifying Genomic Sites of ADP-Ribosylation Mediated by Specific Nuclear PARP Enzymes Using Click-ChIP (Ryan A. Rogge, Bryan A. Gibson, W. Lee Kraus)....Pages 371-387
Front Matter ....Pages 389-389
Methods for Using a Genetically Encoded Fluorescent Biosensor to Monitor Nuclear NAD+ (Michael S. Cohen, Melissa L. Stewart, Richard H. Goodman, Xiaolu A. Cambronne)....Pages 391-414
Correction to: ADP-ribosylation and NAD+ Utilizing Enzymes (Paul Chang)....Pages E1-E1
Back Matter ....Pages 415-417

✦ Subjects

Life Sciences; Biochemistry, general

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