Administration of bone marrow cells into surgically induced fibrocollagenous tunnels induces angiogenesis in ischemic rat hindlimb model

✍ Scribed by Luis Padilla; Edgar Krötzsch; Paul Schalch; Siegfried Figueroa; Adriana Miranda; Enrique Rojas; Sandro Esperante; Fernando Villegas; Anabel S. De La Garza; Mauricio Di Silvio

• Publisher: John Wiley and Sons
• Year: 2003
• Tongue: English
• Weight: 273 KB
• Volume: 23
• Category: Article
• ISSN: 0738-1085
• DOI: 10.1002/micr.10208

✦ Synopsis

Abstract

We established a comparative model of angiogenic induction in previously formed fibrocollagenous tunnels in rat inner thigh muscles. A unilateral hindlimb chronic ischemia model was performed in male Sprague‐Dawley rats. A device was then inserted in the central portion of the inner thigh muscles. Vascularity in the ischemic limb was determined by means of an angiographic score, capillary/fiber ratio, and endothelial proliferation by histochemistry and immunohistochemistry. Autologous transplant of bone marrow, vascular endothelial growth factor (VEGF), or collagen‐polyvinylpyrrolidone plus heparin induced significant vascularization of the ischemic hindlimb when compared to saline solution. However, the bone marrow group presented a higher angiographic score than the other two. No differences among groups were observed in capillary/fiber ratio or proliferation, except for the VEGF group, where capillary proliferating cells were significantly higher than in controls. Based on these results, bone marrow‐derived progenitor cells may constitute a safe and viable alternative for the induction of therapeutic angiogenesis. © 2003 Wiley‐Liss, Inc. MICROSURGERY 23:568–574 2003