Adipose differentiation of bone marrow-derived mesenchymal stem cells using Pluronic F-127 hydrogel in vitro

✍ Scribed by Aditya V. Vashi; Efthimia Keramidaris; Keren M. Abberton; Wayne A. Morrison; Jeremy L. Wilson; Andrea J. O’Connor; Justin J. Cooper-White; Erik W. Thompson

• Publisher: Elsevier Science
• Year: 2008
• Tongue: English
• Weight: 796 KB
• Volume: 29
• Category: Article
• ISSN: 0142-9612
• DOI: 10.1016/j.biomaterials.2007.10.017

✦ Synopsis

Due to increasing clinical demand for adipose tissue, a suitable scaffold for engineering adipose tissue constructs is needed. In this study, we have developed a three-dimensional (3-D) culture system using bone marrow-derived mesenchymal stem cells (BM-MSC) and a Pluronic F-127 hydrogel scaffold as a step towards the in vitro tissue engineering of fat. BM-MSC were dispersed into a Pluronic F-127 hydrogel with or without type I collagen added. The adipogenic differentiation of the BM-MSC was assessed by cellular morphology and further confirmed by Oil Red O staining. The BM-MSC differentiated into adipocytes in Pluronic F-127 in the presence of adipogenic stimuli over a period of 2 weeks, with some differentiation present even in absence of such stimuli. The addition of type I collagen to the Pluronic F-127 caused the BM-MSC to aggregate into clumps, thereby generating an uneven adipogenic response, which was not desirable.