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Adipose angiogenesis: quantitative methods to study microvessel growth, regression and remodeling in vivo

✍ Scribed by Xue, Yuan; Lim, Sharon; Bråkenhielm, Ebba; Cao, Yihai


Book ID
109946864
Publisher
Nature Publishing Group
Year
2010
Tongue
English
Weight
860 KB
Volume
5
Category
Article
ISSN
1750-2799

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We describe a quantitative assay for assessing tumor angiogenesis in vivo using basement membrane extracts (Matrigel). Nude mice were injected S.C. with liquid Matrigel mixed with HT I080 human fibrosarcoma cells. Since Matrigel rapidly forms a solid gel at body temperature, the gel containing tumor