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Adipogenic activity produced by hepatocyte-derived cell lines and by normal hepatocytes in primary culture

✍ Scribed by Hirokazu Zaitsu; Ginette Serrero

Publisher: John Wiley and Sons
Year: 1991
Tongue: English
Weight: 860 KB
Volume: 149
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1041490223

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Culture media conditioned by several hepatocyte derived cell lines were analyzed for their ability to stimulate adipose differentiation of the adipogenic cell line 1246. The results presented here show that culture media from HepG2 and Hep3B cell lines contain a high level of the activity, whereas media from hepatoma and hepato adenocarcinoma cell lines Huh‐7, PLC/PRF/5, and SKHep‐1 do not contain adipogenic activity. Conditioned medium from HepG2 cells also stimulated differentiation of 3T3‐L~1~ cells and of rat epididymal adipocyte precursors in primary culture. Partial biochemical characterization of the adipogenic activity carried out using HepG2 conditioned medium indicates that the hepatocyte derived adipogenic factor has an apparent molecular weight between 445 and 232 kDa, is destroyed by treatment at 100°C, with protease, with 2‐mercaptoethanol and in acidic conditions. The activity is stable at alkaline pH. Culture media conditioned by normal rat hepatocytes in primary culture also contained adipogenic activity. In contrast, medium conditioned by primary culture of nonhepatocyte cells also isolated from liver was deprived of this activity. The data presented in this paper suggest that hepatocytes could be a physiological site of production of adipogenic activity.

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