Adhesion and growth of dental pulp stem cells on enamel-like fluorapatite surfaces

✍ Scribed by J. Liu; T. C. Jin; S. Chang; A. Czajka-Jakubowska; B. H. Clarkson

• Publisher: John Wiley and Sons
• Year: 2011
• Tongue: English
• Weight: 536 KB
• Volume: 96A
• Category: Article
• ISSN: 1549-3296
• DOI: 10.1002/jbm.a.33002

✦ Synopsis

Abstract

To study how apatite crystal alignment of an enamel‐like substrate affects DPSC cellular adhesion and growth as a precursor to produce an in vitro enamel/dentin superstructure for future studies. The cells were subcultured in 10% FBS DMEM up to seven weeks on the two surfaces. Specimens were observed under SEM, counted, and analyzed using the human pathway‐focused matrix and adhesion PCR array. After three days, the cell number on ordered FA surface was significantly higher than on the disordered surface. Of the 84 focused pathway genes, a total of 20 genes were either up or down regulated in the cells on ordered FA surface compared to the disordered surface. More interestingly, of the cell‐matrix adhesion molecules, integrin alpha 7 and 8 (ITGA 7 and 8), integrin beta 3 and 4 (ITGB3 and 4), and the vitronectin receptor‐integrin alpha V (ITGAV) and the key adhesion protein‐fibronectin1 (FN1) were up‐regulated. In SEM, both surfaces showed good biocompatibility and supported long term growth of DPSC cells but with functional cell‐matrix interaction on the ordered FA surfaces. Significance: The enhanced cellular response of DPSC cell to the ordered FA crystal surface involves a set of delicately regulated matrix and adhesion molecules which could be manipulated by treating the cells with a dentin extract, to produce a dentin/enamel superstructure. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2011.