Adenoviral vector-directed expression of neurotrophin-3 in rat dorsal root ganglion explants results in a robust neurite outgrowth response
✍ Scribed by Dijkhuizen, Paul A. ;Hermens, Wim T. J. M. C. ;Teunis, Marc A. T. ;Verhaagen, Joost
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 303 KB
- Volume
- 33
- Category
- Article
- ISSN
- 0022-3034
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✦ Synopsis
The neurotrophins are a family of plaque-forming units induced the formation of a dense proteins that promote neuronal survival and neurite halo of neurites comparable to outgrowth observed outgrowth during development and can also enhance the regeneration of injured adult neurons. The local following the addition of 100 ng/mL exogenous NT-3. and continuous delivery of these proteins at the site In addition, a single infection with Ad-NT-3 produced of injury is problematic, since this requires repeated biologically active NT-3 for at least 20 days in culture, intraparenchymal injections or the use of invasive caas evidenced by continued neurite extension. This innula-micropump devices. In the present study we redicates that adenoviral vector-mediated expression of port the generation and characterization of an adeno-NT-3 results in high-level production of biologically viral vector for a member of the neurotrophins, neuroactive NT-3 and could therefore be used as a strategy trophin-3 (Ad-NT-3). Using Ad-NT-3, we examined to promote the regeneration of injured peripheral and the expression and biological activity of NT-3 in dorsal central nerve projections. ᭧ 1997 John Wiley & Sons, Inc. root ganglia (DRG) explant cultures. Gene transfer