Adenine Nucleotide Hydrolysis in Patients with Aseptic and Bacterial Meningitis
✍ Scribed by Aracélli Gnatta Dorneles; Charlene Menezes; Rita Leal Sperotto; Marta Medeiros Frescura Duarte; Vera Maria Morsch; Maria Rosa Chitolina Schetinger; Vânia Lúcia Loro
- Book ID
- 106484132
- Publisher
- Springer
- Year
- 2008
- Tongue
- English
- Weight
- 334 KB
- Volume
- 34
- Category
- Article
- ISSN
- 0364-3190
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Between August and November 1985, 45 patients with suspected aseptic meningitis were investigated using conventional virus isolation procedures and the mu-antibody capture Coxsackie B IgM enzyme-linked immunoabsorbent assay (ELISA) test, which is well known to cross-react with other members of the e
## Abstract An assay based on the polymerase chain reaction (PCR) for detection of enteroviral RNA in stool samples was carried out using specimens from 74 patients with aseptic meningitis. The primer pair and probe were derived from the highly conserved 5′ non‐coding enterovirus genomic region. En
## Abstract Two polymerase chain reaction (RT‐PCR) assays were developed to allow rapid detection of enteroviral RNA in cerebrospinal fluid samples (CSF). Primers homologous to the conserved 5′ noncoding region of the enterovirus genome were designed. The RT‐PCR product size was‐500 bp (479 bp for