Additional evidence implicatingTriticum searsiias the B-genome donor to wheat

✍ Scribed by J. Nath; James J. Hanzel; James P. Thompson; James W. McNay

• Publisher: Springer
• Year: 1984
• Tongue: English
• Weight: 646 KB
• Volume: 22
• Category: Article
• ISSN: 0006-2928
• DOI: 10.1007/bf00499285

✦ Synopsis

In vitro DNA.'DNA hybridizations and hydroxyapatite thermal-elution chromatography were employed to identify the diploid wheat species ancestral to the B genome ofTriticum turgidum. 3H-T. turgidum DNA was hybridized to the unlabeled DIVAs ofT. urartu, T. speltoides, T. sharonensis, T. bicorne, T. longissimum, and T. searsii. 3H-Labeled DNAs of T. monococcum and a synthetic tetraploid AADD were hybridized with unlabeled DNAs of T. urartu and T. searsii to determine the relationship of the A genome of polyploid wheat and T. urartu. The heteroduplex thermal stabilities indicated that T. searsii was most closely related to the B genome ofT. turgidum (AB) and that the genome ofT. urartu and the A genome have a great deal of base-sequence homology. Thus, it appears that T. searsii is the B-genome donor to polyploid wheat or a major chromosome donor if the B genome is polyphyletic in origin.