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Addition of Mononucleotides to Oligoribonucleotide Acceptors with T4 RNA ligase

โœ Scribed by Yo Kikuchi, Fumio Hishinuma and Kenji Sakaguchi


Book ID
123654984
Publisher
National Academy of Sciences
Year
1978
Tongue
English
Weight
654 KB
Volume
75
Category
Article
ISSN
0027-8424

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The use of high-performance liquid chrom
โœ Larry W. McLaughlin; Elena Romaniuk ๐Ÿ“‚ Article ๐Ÿ“… 1982 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 664 KB

High-performance liquid chromatography (HPLC) with both amino-propyl-silyl ion-exchange and octadecasilyl reverse-phase columns has been used to simultaneously analyze all substrates, intermediates, and products involved in the T4 RNA ligase reaction. Both singlestep ligations (e.g., UpApA + pCp) as