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Adaptive nonlinear cell mass state estimator for a continuous yeast fermentation

✍ Scribed by Michael E. Ramseir; Pramod Agrawal; Duncan A. Mellichamp


Publisher
American Institute of Chemical Engineers
Year
1993
Tongue
English
Weight
986 KB
Volume
39
Category
Article
ISSN
0001-1541

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✦ Synopsis


On-line measurement of the important state variables in fermentations, particularly cell mass concentration, remains a difficult problem. However, a number of secondary or environmental variables can be measured conventionally and on-line, such as pH, and C02 and 0, in the exhaust gas. Stephanopoulos and San (1984) have developed a modeling approach, based on species balances, that provides relations between the environmental and important state variables. Using such a model, the important state variables can be estimated in principle from more easily accessible on-line measurements.

In this article, a new adaptive estimator is developed, incorporating as its basis

an underlying nonlinear model so as to utilize the best possible a priori process knowledge. Base addition rate and COz offgas concentration are measured on-line and periodically. Cell mass measurements are incorporated in frequently and even at irregular sampling periods, thus providing a very flexible scheme. Only a single adapted parameter is required to match the model to the plant operating characteristics. This simple but rigorous model form results in an estimator that is easy to implement and to tune and which exhibits long-term robustness due to its multirate feedback structure. Experimental results from a laboratory-scale continuous fermentor show that such a cell mass estimation scheme yields excellent performance both open-loop (without control) and as a part of conventional and nonlinear adaptive control approaches.