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Adaptation of Chinese hamster ovary cells to low culture temperature: Cell growth and recombinant protein production

โœ Scribed by Sung Kwan Yoon; Jong Kwang Hong; Seung Ho Choo; Ji Yong Song; Hong Woo Park; Gyun Min Lee

Book ID
118063017
Publisher
Elsevier Science
Year
2006
Tongue
English
Weight
465 KB
Volume
122
Category
Article
ISSN
0168-1656

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The use of a temperature switch to control the growth and productivity of temperature-sensitive (ts) mutants was investigated to extend the productive life span of recombinant Chinese hamster ovary (CHO) cells in batch culture. Bromodeoxyuridine was used at 39ยฐC to select mutagenized CHO-K1 cells, w