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Acyl transfer reactions catalyzed by native and modified α-chymotrypsin in acetonitrile with low water content

✍ Scribed by Václav Čeěovský; Hans-Dieter Jakubke


Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
607 KB
Volume
16
Category
Article
ISSN
0141-0229

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Nucleophile specificity of subtilisin in
✍ Václav Čeřovský; Hans-Dieter Jakubke 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 581 KB

Nucleophile specificity of subtilisin (subtilopeptidase A) was studied via acyl transfer reactions in acetonitrile containing piperidine and 10 vol% of water. Ac-Tyr-OEt was used as acyl donor and a series of amino acid derivatives, diand tripeptides of the general structure Xaa-Gly, Gly-Xaa, Gly-Gl