Nucleophile specificity of subtilisin in
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Václav Čeřovský; Hans-Dieter Jakubke
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Article
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2000
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John Wiley and Sons
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English
⚖ 581 KB
Nucleophile specificity of subtilisin (subtilopeptidase A) was studied via acyl transfer reactions in acetonitrile containing piperidine and 10 vol% of water. Ac-Tyr-OEt was used as acyl donor and a series of amino acid derivatives, diand tripeptides of the general structure Xaa-Gly, Gly-Xaa, Gly-Gl