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Activity, stability, and unfolding of reconstituted horseradish peroxidase with modified heme

✍ Scribed by Hai-Yan Song; Jian-Zhong Liu; Li-Ping Weng; Liang-Nian Ji


Publisher
Elsevier Science
Year
2009
Tongue
English
Weight
592 KB
Volume
57
Category
Article
ISSN
1381-1177

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## Abstract Phthalic anhydride (PA) modification stabilizes horseradish peroxidase (HRP) by reversal of the positive charge on two of HRP's six lysine residues. Native and PA‐HRP had half‐inactivation temperatures of 51 and 65Β°C and half‐lives at 65Β°C of 4 and 17 min, respectively. PA‐HRP was more