A broad-host-range vector, pKT240, containing the structural gene (aph) for aminoglycoside phosphotransferase (APH), without promoter, has been constructed. Insertion of DNA fragments carrying promoters upstreamof the aph gene into the unique EcoRI site of this vector results in the expression of th
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Activity of the hybrid trp-lac (tac) promoter of Escherichia coli in Pseudomonas putida. Construction of broad-host-range, controlled-expression vectors
✍ Scribed by Miroslawa M. Bagdasarian; Egon Amann; Rudolf Lurz; Beate Rückert; Michael Bagdasarian
- Book ID
- 118174969
- Publisher
- Elsevier Science
- Year
- 1983
- Tongue
- English
- Weight
- 964 KB
- Volume
- 26
- Category
- Article
- ISSN
- 0378-1119
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To determine the utility of coupling runaway replication to the expression of cloned genes under the control of strong promoters, lacZ transcriptional fusions to the trp or tac promoter (Ptrp or Ptac) were constructed using plasmids in which the copy number is thermally regulated. Cells containing t