Activity of the cytochrome P450 enzyme geraniol 10-hydroxylase and alkaloid production in plant cell cultures
β Scribed by Graziella Collu; Aurelio Alonso Garcia; Robert van der Heijden; Robert Verpoorte
- Book ID
- 117382669
- Publisher
- Elsevier Science
- Year
- 2002
- Tongue
- English
- Weight
- 378 KB
- Volume
- 162
- Category
- Article
- ISSN
- 0168-9452
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The cytochrome P450 enzyme geraniol 10-hydroxylase plays an important role in the biosynthesis of pharmaceutically important alkaloids in Catharanthus roseus. An HPLC assay was developed for this enzyme based on the UV detection of the product 10-hydroxygeraniol after its separation from the substra
Cell suspension cultures of alfalfa (Medicago sativa L.) accumulated phenolic secondary metabolites in a pattern similar to that seen in alfalfa roots. Upon treatment with a crude elicitor preparation from the bean pathogen Colletotrichum lindemuthianum, the pterocarpan phytoalexin medicarpin accumu