Activity of Immobilized Trypsin in the Layer Structure of Polyelectrolyte Microcapsules (PEMC)
✍ Scribed by Eike Garbers; Rita Mitlöhner; Radostina Georgieva; Hans Bäumler
- Publisher
- John Wiley and Sons
- Year
- 2007
- Tongue
- English
- Weight
- 195 KB
- Volume
- 7
- Category
- Article
- ISSN
- 1616-5187
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✦ Synopsis
Abstract
Polyelectrolyte microcapsules composed by using the LbL technique on stabilized RBC as templates were coated with up to ten layer pairs of trypsin/PSS or trypsin/alginate. The trypsin layer growth was confirmed by particle electrophoresis, confocal laser scanning microscopy, flow cytometry, and protein determination according to Lowry. In the coating series with trypsin/PSS, the amount of immobilized enzyme was larger than that with trypsin/alginate. The enzyme immobilization led to activity reduction of up to 90% compared to that of the same enzyme amount in the solution. No significant differences between the activities of trypsin immobilized in combination with PSS and with alginate were found.
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