Activity of branched dextrans in the acceptor reaction of a glucosyltransferase (GTF-I) from Streptococcus mutans OMZ176
✍ Scribed by Gwen J. Walker; Conrad Schuerch
- Book ID
- 102991131
- Publisher
- Elsevier Science
- Year
- 1986
- Tongue
- English
- Weight
- 889 KB
- Volume
- 146
- Category
- Article
- ISSN
- 0008-6215
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✦ Synopsis
The ability of several native and chemically synthesized, branched dextrans to stimulate the activity of an a-D-glucosyltransferase (GTF-I) of Streptococcus mutuns has been compared. The enzyme catalysed the transfer of glucosyl residues from sucrose with the formation of water-insoluble (1+3)-a-D-glucan. The rate of this reaction was greatly increased in the presence of dextran, and the extent of stimulation was negatively correlated with the degree of branching of the added dextran. The results refute the concept that growth of water-insoluble glucan occurs from the multiple, non-reducing termini of dextran acceptors.
*Dedicated to Professor L. F. Leloir on the occasion of his 80th birthday.