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Active-site residue, domain and module swaps in modular polyketide synthases

✍ Scribed by Francesca Del Vecchio; Hrvoje Petkovic; Steven G. Kendrew; Lindsey Low; Barrie Wilkinson; Rachel Lill; Jesús Cortés; Brian A. M. Rudd; Jim Staunton; Peter F. Leadlay

Book ID
106308937
Publisher
Springer-Verlag
Year
2003
Tongue
English
Weight
260 KB
Volume
30
Category
Article
ISSN
1476-5535

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## Abstract Multiple versions of the DEBS 1‐TE gene, which encodes a truncated bimodular polyketide synthase (PKS) derived from the erythromycin‐producing PKS, were created by replacing the DNA encoding the ketoreductase (KR) domain in the second extension module by either of two synthetic oligonuc