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Active Site of Bee Venom Phospholipase A 2 : The Role of Histidine-34, Aspartate-64 and Tyrosine-87 †

✍ Scribed by Annand, Robert R.; Kontoyianni, Maria; Penzotti, Julie E.; Dudler, Thomas; Lybrand, Terry P.; Gelb, Michael H.

Book ID
126087463
Publisher
American Chemical Society
Year
1996
Tongue
English
Weight
466 KB
Volume
35
Category
Article
ISSN
0006-2960

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Trimeresurus fivoviridis (Habu snake) venom aspartate-49-phospholipase A2 (Asp-49-PLAz) was reacted at pH 9.0 with a 2-fold molar excess of 2,4,6-trinitrobenzenesdfonate in the absence of Ca" and two trinitrophenylated derivatives were isolated by HPLC. One was a derivative modified at Lys-11 and it