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Active site histidine in spinach ribulosebisphosphate carboxylase/oxygenase modified by diethyl pyrocarbonate

โœ Scribed by Igarashi, Yasuo; McFadden, Bruce A.; El-Gul, Ted


Book ID
126165366
Publisher
American Chemical Society
Year
1985
Tongue
English
Weight
712 KB
Volume
24
Category
Article
ISSN
0006-2960

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Active-site histidines in recombinant cy
โœ Robert L. Haining; Bruce A. McFadden ๐Ÿ“‚ Article ๐Ÿ“… 1994 ๐Ÿ› Springer ๐ŸŒ English โš– 903 KB

The functions of H i s 291 , H i s 295 and H i s 324 at the active-site of recombinant A. nidulans ribulose-1,5-bisphosphate carboxylase/oxygenase have been explored by site-directed mutagenesis. Replacement of His 291 by K or R resulted in unassembled proteins, while its replacement by E, Q or N re