Active oxygen transforms murine myeloid progenitor cells in vitro

✍ Scribed by Dana R. Crawford; Joel S. Greenberger

• Book ID: 102864179
• Publisher: John Wiley and Sons
• Year: 1991
• Tongue: French
• Weight: 793 KB
• Volume: 49
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910490519

✦ Synopsis

Active oxygen (AO) is ubiquitous in nature and its many forms can act as natural carcinogens. Their effect on the transformation of a mouse myeloid progenitor cell line was studied using anchorage-independent colony formation in methylcellulose as the primary assay. Both cytotoxic and non-toxic concentrations of t-butylhydroperoxide, hydrogen peroxide and menadione were examined. At non-cytotoxic concentrations, no A 0 transformation of these cells from interleukin-3 dependence to factor independence (FI) was observed, even after as many as 25 treatments. At cytotoxic concentrations, however, all 3 classes of A 0 transformed the cells to FI growth. The most potent agent was t-butyl hydroperoxide (43-fold induction), followed 'To whom correspondence and reprint requests should be addressed.