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Activation of transcription of a yeast gene in E. coli by an IS5 element

✍ Scribed by Jund, Richard; Loison, Gérard


Book ID
109726164
Publisher
Nature Publishing Group
Year
1982
Tongue
English
Weight
260 KB
Volume
296
Category
Article
ISSN
0028-0836

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The 5′-upstream region of the yeast 25S
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The 25S rRNA gene of Saccharomyces cerevisiae is preceded by a bona fide TATA sequence which allows the initiation of transcription--presumably by polymerase II--from the same strand as the 25S rRNA gene. When the promoter fragment is cloned in front of a lacZ gene equipped with an initiation codon