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Activation of the Sarcophaga lectin gene promoter in transgenic Drosophila

✍ Scribed by Takahiro Tanji; Ayako Kobayashi; Shunji Natori

Publisher: John Wiley and Sons
Year: 2002
Tongue: English
Weight: 200 KB
Volume: 50
Category: Article
ISSN: 0739-4462
DOI: 10.1002/arch.10039

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✦ Synopsis

We established transgenic Drosophila strains in which the lacZ gene was expressed under the control of the 5'-upstream regulatory region of the Sarcophaga lectin gene promoter (3.1 kbp). The reporter gene was expressed in the fat bodies of the transgenic larvae when they were immunized by body pricking or treatment with Escherichia coli, which was the same as the Sarcophaga lectin gene expression in Sarcophaga larvae. However, the same reporter gene was found to be expressed constitutively in the digestive tracts of the transgenic larvae even without immunization.

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