Activation of the hypothalamo-anterior pituitary corticotropin-releasing hormone, adrenocorticotropin hormone and β-endorphin systems during the estradiol 17β-induced plasma LH surge in the ovariectomized monkey
✍ Scribed by B. Kerdelhué; G. S. Jones; K. Gordon; H. Seltman; V. Lenoir; S. Mélik Parsadaniantz; R. F. Williams; G. D. Hodgen
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 704 KB
- Volume
- 42
- Category
- Article
- ISSN
- 0360-4012
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✦ Synopsis
The present work describes time-dependent changes in the content of corticotropin-releasing hormone (CRH), adrenocorticotropin (ACTH), and P-endorphin (P-EP) in the hypothalamus (HT) and anterior pituitary (AP) and in the concentration of ACTH and P-EP in the plasma during the 17P estradiol (E,) benzoate (E,B)-induced luteinizing hormone (LH) surge in ovariectomized cynomolgus monkeys. Monkeys were euthanized at 0, 30,48,72, and 96 hr post-E,B. HT and AP were rapidly dissected, extracted in 2 N acetic acid containing 1 mM phenylmethane sulfonyl fluoride at 4°C. and centrifuged at 18,OOOg for 30 min. Peptide concentrations were measured in the supernatant by specific radioimmunoassays (RIAs).
In the HT, there were significant (P < 0.05) decreases