Activation of polyester cloth for the immobilization of a polysaccharide antigen
โ Scribed by Burton W. Blais; Hiroshi Yamazaki
- Book ID
- 104647184
- Publisher
- Springer-Verlag
- Year
- 1990
- Tongue
- English
- Weight
- 259 KB
- Volume
- 4
- Category
- Article
- ISSN
- 0951-208X
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โฆ Synopsis
A polysaccharide antigen was immobilized on polyester cloth using cyanuric chloride as a coupling agent, yielding an antigen-coated solid phase suitable for enzyme immunoassay. The method should be generally useful for coating polyester cloth with amino or hydroxyl containing antigens or haptens which otherwise adsorb poorly to the cloth.
๐ SIMILAR VOLUMES
A non-woven rayon/polyester cloth was treated with NaOH and activated with 1,l -carbonyldiimidazole. The activated cloth was washed and used without drying to immobilize bovine serum albumin. Drying of the cloth markedly decreased protein immobilization. The advantages of using the activated clot