Activation of gef-h1, a guanine nucleotide exchange factor for RhoA, by DNA transfection
✍ Scribed by Marcus Brecht; Agatha C.M. Steenvoorden; John G. Collard; Susanne Luf; Dorothee Erz; Claus R. Bartram; Johannes W.G. Janssen
- Publisher
- John Wiley and Sons
- Year
- 2004
- Tongue
- French
- Weight
- 294 KB
- Volume
- 113
- Category
- Article
- ISSN
- 0020-7136
No coin nor oath required. For personal study only.
✦ Synopsis
Several oncogenes isolated by the NIH/3T3 transformation assay, i.e., dbl, dbs, lbc, lfc, lsc, net, ost and tim, contain a Dbl homology (DH) and a pleckstrin-homology (PH) domain and act as GEFs (guanine nucleotide exchange factors) for Rho-like GTPases. In a search for genes with oncogenic potential in DNA from the monocytic leukaemia cell line U937, we identified an amino-terminal truncated form of gef-h1, a gene encoding a GEF for RhoA. These data support the idea that a systematic search for mutations and/or deletions of GEFs in human cancer is promising.