Activation of Cellulose Membranes with 1,1′-Carbonyldiimidazole or 1-Cyano-4-dimethylaminopyridinium tetrafluoroborate as a Basis for the Development of Immunosensors

✍ Scribed by Daniela Stöllner; Frieder W. Scheller; Axel Warsinke

• Book ID: 102562227
• Publisher: Elsevier Science
• Year: 2002
• Tongue: English
• Weight: 87 KB
• Volume: 304
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1006/abio.2001.5603

✦ Synopsis

Methods for the activation of a cellulose dialysis membrane for immunosensor applications have been developed. For activation two reagents, 1,1carbonyldiimidazole (CDI) and 1-cyano-4-dimethylaminopyridinium tetrafluoroborate (CDAP), were compared with respect to the coupling efficiency for glucose oxidase (GOx) and 1,8-diamino-2,6-dioxaoctane. The maximum level of activation was 2.4 mol cm ؊2 for CDI and 0.2 mol cm ؊2 for CDAP activation. We observed 1.5 g cm ؊2 and 0.4 ؋ 10 ؊4 U cm ؊2 GOx with CDI-activated membranes whereas 1.7 g cm ؊2 and 7.2 ؋ 10 ؊4 U cm ؊2 GOx were observed with CDAP-activated membranes. With 1,8-diamino-2,6dioxaoctane amino group densities of 0.165 and 0.09 mol cm ؊2 were observed via CDI and CDAP activation, respectively. An amino-modified membrane was used for coupling a ligand (pentapeptide) and an immunoenzymometric assay for hemoglobin A1c was carried out.