Production of large amounts of 35S RNA a
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R.E. Smith; S. Nebes; J. Leis
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Article
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1977
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Elsevier Science
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English
β 533 KB
An automatic perfusion device was used to collect virus-containing supernatant fluids from transformed and untransformed chick embryo fibroblasts every 2 hr. A 30-day perfusion of 10 roller culture bottles of Rous sarcoma virusinfected cells yielded 520 mg of virus and 18 x lo9 transformed cells. Qu