𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Activation of Cdk2 is a requirement for antigen-mediated thymic negative selection

✍ Scribed by Owen Williams; Gabriel Gil-Gómez; Trisha Norton; Dimitris Kioussis; Hugh J. M. Brady

Publisher
John Wiley and Sons
Year
2000
Tongue
English
Weight
89 KB
Volume
30
Category
Article
ISSN
0014-2980

No coin nor oath required. For personal study only.

✦ Synopsis

Apoptosis plays a critical role in T cell development and thymic selection. Thymocytes which undergo antigen-induced negative selection have been demonstrated to die by apoptosis. Despite this, relatively little is known about the specific apoptotic pathway involved in negative selection. We have examined the role of cyclin-dependent kinase 2 (Cdk2), a key regulator of thymocyte apoptosis, in this process. Stimulation of thymocytes with cognate antigen leads to a large increase in Cdk2 kinase activity. We also show that pharmacological inhibitors of Cdk2 block thymocyte apoptosis in response to antigen. Our data show that Cdk2 activity is essential for the apoptotic pathway used in negative selection.

📜 SIMILAR VOLUMES

Sodium butyrate-mediated induction of th
Sodium butyrate-mediated induction of the glycoprotein hormone α-subunit gene: Requirement for continued protein synthesis, identification of a butyrate-responsive element, and inhibition of promoter activation by 2-deoxyglucose
✍ Michael J. Haas; Dominic E. Cosgrove; Wanfen Xiong; G. Stanley Cox 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 420 KB

Production of the glycoprotein hormone ␣-subunit (GPH␣) was enhanced by sodium butyrate (Btr) in HeLa cells. Induction of the HeLa ␣-subunit gene by Btr was inhibited by the simultaneous addition of cycloheximide (CHX), indicating a requirement for continued protein synthesis. Transient expression a