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Activation and inhibition processes of alkaline phosphatase from bovine brain by metal ions (Mg2+ and Zn2)

✍ Scribed by Claude Brunel; Guy Cathala


Book ID
115733336
Publisher
Elsevier Science
Year
1973
Weight
655 KB
Volume
309
Category
Article
ISSN
0005-2744

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Alkaline phosphatase (EC 3.1.3.1) from bovine intestinal mucosa (BIAP) is an homodimeric metalloenzyme, containing one Mg 2Ψ‰ and two Zn 2Ψ‰ ions in each active site. ApoBIAP, prepared using ion-chelating agents, exhibited a dramatic decrease of its hydrolase activity, concomittant to conformational c