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Activating the Prokaryotic leu-500 Promoter by Transient, Dynamic DNA Supercoiling in Escherichia Coli

✍ Scribed by Zhi, Xiaoduo; Perez, Catherine; Dages, Samantha; Dages, Kelley; Eichelbaum, Steve; Makemson, John; Leng, Fenfei


Book ID
123224644
Publisher
Biophysical Society
Year
2014
Tongue
English
Weight
135 KB
Volume
106
Category
Article
ISSN
0006-3495

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We have investigated in vivo the coupling between CytR regulation of the deoP2 promoter in Escherichia coli and the DNA-binding specificity of the cAMP-CRP (cAMP receptor protein) complex in order to obtain a more detailed picture of the role played by cAMP-CRP in CytR regulation. By introducing CRP