Isolation, purification, and quantitatio
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Mary J. Ruwart; Donald L. Kaminski
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Article
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1977
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Elsevier Science
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English
β 323 KB
A method has been established to purify and separate cyclic AMP and cyclic GMP from canine bile. Perchloric acid extracts are treated with Dowex 1 resin. This batch processing binds the nucleotides completely. but very little biliary pigment (less than 5%). The Dowex I resin is then layered onto a D