Action of recombinant alpha interferon against experimental and spontaneous metastases in a murine model

✍ Scribed by P. Ramani; F. R. Balkwill

• Publisher: John Wiley and Sons
• Year: 1989
• Tongue: French
• Weight: 675 KB
• Volume: 43
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910430126

✦ Synopsis

The therapeutic potential of rHulFN-a A/D, a hybrid human IFN molecule with equal activity on murine cells, was studied in experimental and spontaneous metastatic models of a murine colon carcinoma COLON 26. rHulFN-a! A/D inhibited experimental pulmonary metastases of COLON 26 and prolonged the survival of BALB/c mice. Dose scheduling, survival and tumour-cell clearance studies showed that the first 5 days were critical in the inhibition of pulmonary metastases. However, it is unlikely that lung N K cells were involved in the anti-metastatic effect of rHulFN-a AID because inhibition of pulmonary metastases and a decrease in radio-labelled tumour-cell survival was seen in BALBlc mice depleted selectively of their NK cells by prior treatment with rabbit anti-asialoGM, serum. Although rHuIFN-a A/D stimulated NK-cell activity in BALB/c mice, it was ineffective in abrogating the NK suppressant action of rabbit anti-asialoGMl serum on murine lung NK cells. Thus, IFN may mediate its early antimetastatic effect via a mechanism independent of NK-cell stimulation. IFN also inhibited the development of lung metastases from S.C. COLON 26 tumours in normal, NK-depleted and T-cell-deficient mice.